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op puro based crispr cas9 screen  (Addgene inc)


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    Structured Review

    Addgene inc op puro based crispr cas9 screen
    Op Puro Based Crispr Cas9 Screen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/op+puro+based+crispr+cas9+screen/Puro-Cas9+donor+(Plasmid+%2358409)/pm39198484-331-0-23
    Average 94 stars, based on 139 article reviews
    op puro based crispr cas9 screen - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    CRISPR:

    Article Title: P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance.
    Article Snippet: .. OP-Puro based CRISPR-Cas9 screen in BJ-Ras-ER cells sgRNAs targeting all human translation factors (Supplementary Fig. 2a) were cloned into the lentiCRISPR v2-Puro vector (Addgene, 52961) at the BsmBI site. .. To generate viruses, HEK-293T cells were co-transfected with the following vectors: VSV-G (Addgene, 8454), pMDLg/pRRE (Addgene, 12251), pRSV-Rev (Addgene, 12253), and lentiCRISPR v2-Puro containing the sgRNAs, using JetPrime Transfection Reagent (Polyplus).

    Clone Assay:

    Article Title: P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance.
    Article Snippet: .. OP-Puro based CRISPR-Cas9 screen in BJ-Ras-ER cells sgRNAs targeting all human translation factors (Supplementary Fig. 2a) were cloned into the lentiCRISPR v2-Puro vector (Addgene, 52961) at the BsmBI site. .. To generate viruses, HEK-293T cells were co-transfected with the following vectors: VSV-G (Addgene, 8454), pMDLg/pRRE (Addgene, 12251), pRSV-Rev (Addgene, 12253), and lentiCRISPR v2-Puro containing the sgRNAs, using JetPrime Transfection Reagent (Polyplus).

    Plasmid Preparation:

    Article Title: P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance.
    Article Snippet: .. OP-Puro based CRISPR-Cas9 screen in BJ-Ras-ER cells sgRNAs targeting all human translation factors (Supplementary Fig. 2a) were cloned into the lentiCRISPR v2-Puro vector (Addgene, 52961) at the BsmBI site. .. To generate viruses, HEK-293T cells were co-transfected with the following vectors: VSV-G (Addgene, 8454), pMDLg/pRRE (Addgene, 12251), pRSV-Rev (Addgene, 12253), and lentiCRISPR v2-Puro containing the sgRNAs, using JetPrime Transfection Reagent (Polyplus).



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    94
    Addgene inc op puro based crispr cas9 screen
    Op Puro Based Crispr Cas9 Screen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/op+puro+based+crispr+cas9+screen/Puro-Cas9+donor+(Plasmid+%2358409)/pm39198484-331-0-23
    Average 94 stars, based on 1 article reviews
    op puro based crispr cas9 screen - by Bioz Stars, 2026-10
    94/100 stars
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